Susquehanna Valley Section

April 2002 Meeting

The three hundred and thirty fifth meeting of the Section will be held at College Misericordia, in Dallas, PA, on Wednesday, April 3, 2002. The speaker will be Hua Jiang, Ph.D., Senior Scientist at Wyeth-Lederle Laboratories.

Dinner will be at "STAX & Co" Restaurant on Route 415 in Dallas. Social Hour 5:15, Dinner 6:15. Call Helen Bogdon, Science Secretary (570) 674-6378 by Monday, April 1^st, for reservations.

Directions: TO Misericordia From I-81 Exit 170B (old exit 47B) take Route 309 North. Follow Route 309 across the river, about 4-5 miles, (signs say "Dallas") and up the steep hill on the other side about 3-4 miles. At the five-way intersection (the seventh or eighth light) take the second RIGHT. Look carefully. There is a sign for "Misericordia". This is Lake St. The five-way intersection is 100 yards past a Wendy's on the right. From Lake St., go straight at the Stop sign. Misericordia's main gate is 100 yards further on the right. Once on the campus, go up the driveway, bear right, park next to the Science building which is the second building on the right, the all glass building very near the road.
TO Misericordia From the West off of I-80 Take I-80 to the Bloomsburg/Lightstreet (239) exit Rt. 487 North. Go north (15-20 miles) to Route 118 then RIGHT on Rt.118 East for about 20-22 miles to the end (in Dallas). At this point go RIGHT on Route 415. About two miles down at the first light (Center Hill Rd.) go LEFT. Take the next LEFT at the stop sign. Misericordia main gate is 100 yards further on the right. Once on the campus, follow the above directions.

For "Stax & Co" restaurant: From I-81 Follow directions from I-81 as above. Do NOT turn on Lake St. Stay on main road (in Dallas, this becomes Route 415.) "Stax" restaurant is on the LEFT side of Route 415 about a mile north of Lake St (and Wendy's).
For "Stax & Co" from the West off of I-80 Follow directions from I-80 above. "Stax" restaurant is on RIGHT side of Route 415 a mile before the Turn on Center Hill Rd.

The meeting will begin at approximately 7:30 pm.

"Production of Recombinant Proteins for Medicinal Application"
Recombinant proteins, such as monoclonal antibodies, cytokines, enzymes and vaccines, are commonly used in diagnosis and therapy. Genetic engineering allows the proteins be expressed in prokaroytes and eukaryotes upon need. These can be purified through different methods to meet the criteria of drug standards. The presentation will briefly review some of the recombinant proteins in drug application, and discuss several details of the protein expression and purification from bacterial, yeast, insect cell, mammalian cell, plant and transgenic animals.

Dr. Hua Jiang obtained her Ph.D. in 1996 under the supervision of Dr. Tom Kodadek in the Dept. of Chemistry and Biochemistry, University of Texas at Austin. For her thesis work she studied the mechanism of DNA homologous recombination in both bacteriophage T4 and yeast Saccharomyces cerevisiae. Dr. Jiang had her postdoctoral training at the National Cancer Institute with Dr. John Brady in the laboratory of Molecular Virology, Virus Tumor Biology Section. Here she discovered a novel interaction between a HTLV-I (Human T-cell Lymphotropic Virus Type I) transactivator with an important cellular transcription regulator. In 1999 Dr. Jiang joined SAIC (Scientific Application International Corp.) in Frederick, MD as a scientist in Process Developing Group of the Biopharmaceutical Development Program. Here she developed methods of protein expression from various hosts and protein purification for clinical trials. Currently Dr Jiang is at Wyeth-Lederle Vaccines as a senior scientist focused on viral vaccine process development.