April 2006 Meeting
The three hundred and sixty third meeting of the Susquehanna Valley Section will be held on Wednesday, April 5, 2006 at 7:45 PM in room > G09 of the Heim Building on the Lycoming College campus. The speaker will be Dr. Jeremy D. Ramsey, Assistant Professor of Chemistry, Lycoming College who will present a talk entitled "Integrated Microfluidic Devices for Sample Preparation and High Efficiency Separations."Dinner will begin at 6:00 pm at the Jonas Room on the campus of Lycoming College. Please email reservations to Jeremy Ramsey (ramsey@lycoming.edu) by April 1.
The meeting and presentation will follow the dinner.
Directions: From I-80, take Route 15 north. Travel approximately 15 miles to Williamsport. Continue over the Market Street Bridge (stay in left lane) and follow the signs for the Business District. Go to the third traffic signal and turn right onto Little League Boulevard. Go 1 block east and turn left at the stop sign onto Mulberry Street. At the next traffic signal, turn right onto Washington Boulevard. The entrance to the Heim Building parking lot will be the first right. We will have guides to direct attendees to the Jonas Room.
Integrated Microfluidic Devices for Sample Preparation and High Efficiency Separations
Work completed at: Geo-Centers, Inc./Naval Research Laboratory; Washington, D.C.
Microfluidic devices have proven to be an effective platform for analytical measurement due to small sample sizes, rapid analysis times, and efficient flow control. These advantages have led to the integration of commonly performed laboratory techniques onto microdevices and spawned the term "laboratory on a chip." The work presented will cover two aspects of analytical microchip measurements, sample processing and multidimensional separations. In the first portion of the presentation, methods for preconcentrating analytes using hydrophobic column packing materials will be discussed. Through selective polymerization of methacrylate monomers, a porous plug that acts as a frit for retaining silica beads can be positioned at any location throughout a microfluidic network. The fabricated columns, when utilized for solid phase extraction, possess high loading capacities, extraction efficiencies, and preconcentration factors. Detection limits for neutral fluorescent molecules are well into the femtomolar range. In the second portion of the presentation, multidimensional separation devices will be discussed. Because of the elegant flow control realized on microfluidic devices, facile coupling of two complementary separations is possible. Using a novel microfluidic device design, peptides eluting from a 20-cm micellar electrokinetic chromatographic (MEKC) separation were repeatedly sampled by a second dimension where they were subsequently separated using free solution capillary electrophoresis. The microchip analysis time is up to four times faster than previously reported column-based 2D separation techniques and at least 25 times faster than 2D-slab gel electrophoresis. The microchip design, operation, throughput, and efficiency for solid phase extraction and multidimensional separations will be discussed.
Jeremy D. Ramsey is an Assistant Professor of Chemistry at Lycoming College. Dr. Ramsey started his career in chemistry by earning a B.S. in chemistry at Clarion University of Pennsylvania. The journey continued at The Ohio State University in Columbus, Ohio, where he completed the Ph. D. program in analytical chemistry through study of electrochemical systems using spectroscopic methods. In an effort to expand his area of expertise, Jeremy moved to Oak Ridge, Tennessee to work at Oak Ridge National Laboratory. There he acquired skills related to biological analysis and microfabrication. This experience led directly to a position with a defense contractor at the Naval Research Laboratory in Washington, DC. His work there was related to the development of methods for sensing explosives and other warfare agents.
.png)
.png)
.png)
